Introduction

The HKU Protein Analysis Facility is a Faculty of Medicine Core Facility at the University of Hong Kong. We provide equipment and expertise to help in the biophysical analysis of proteins. Our service is both within HKU and open to the wider community.

Any queries about usage then please contact Dr. Julian A. Tanner in the Department of Biochemistry, HKU at jatanner@hku.hk.

Equipment Available

Isothermal Titration Calorimetry (Microcal iTC200)

Differential Scanning Calorimetry (Microcal VP-DSC)

Time-resolved Multimode Plate Reading (Thermo Varioskan Flash and BMG Polarstar Omega)

Usage Policy

The HKU Protein Analysis Facility is open to University of Hong Kong research groups where we try to keep a "hands-on" policy to contribute to Research Postgraduate and Post-Doctoral education and training at low cost. For external users please contact Dr. Julian A. Tanner (jatanner@hku.hk) for availability and costs.

Isothermal Titration Calorimetry

ITC measures heats of interactions to characterize macromolecular interactions. We use the technique typically to measure binding constants, enthalpies and entropies of protein-protein and protein-nucleic acid interactions.

Differential Scanning Calorimetry

DSC measures macromolecular stabilities by scanning the heat changes that occur during controlled increase or decrease of temperature. We most often use the technique to observe protein stabilities, protein unfolding, and nucleic acid folding.