Major Research Areas
- Computational analyses of biological sequences, structures and processes. Emphasis is placed on analyses of gene expression and regulation, analyses of protein structures and structural flexibility, and molecular evolutionary studies. Development of methodology is also pursued.
Cell cycle regulation and cancer:
- Regulation of cell cycle progression; altered glycosylation in tumour progression; genomic instability and consequences of DNA-damage; regulation of cellular senescence and early-onset aging using mouse model.
- Structural, functional and chemical approaches to probing molecular interactions and networks to elucidate the mechanisms of disease.
- Generation and comparative analysis of proteomes using 2-D MS/MS or ICAT LC-MS/MS; proteomic changes associated with ER stress, systems biology.
Functional and developmental genomics:
- Determination and analysis of gene function by gene targeting ('knock-out') technology and in ovo electroporation in chick embryos; identifying genes and pathways by comparative analyses of transcriptomes.
Genetics of disorders:
- Analysis and characterization of predisposing genes for common diseases such as degenerative disc disease, Alzheimer's disease and others; identifying genetic modifiers for congenital heart disease; mechanisms of genetic diseases at the biochemical level.
Glycobiology of cell-matrix interactions:
- Growing nerves in glycosaminoglycan-modified environments; towards glycosaminoglycan intervention of respiratory tract inflammation; glycosaminoglycan metabolism in renal stone formation.
Molecular biology of the extracellular matrix:
- Regulation of chondrocyte differentiation and gene expression; identification of extracellular signals, transcription factors and their target genes important for osteochondroprogenitors and cartilage formation; genetic factors for the regulation of bone growth and healing; transgenic mouse models of inherited connective tissue disease in humans; regulation of matrix assembly in cartilage and bone.
Molecular mechanisms of intracellular transportation and diseases:
- Characterization of the functions of intracellular transportation in neurons, melanocytes and chondrocytes; studies of the relationship between intracellular transportation and Alzheimer's Disease.
Molecular developmental biology:
- Genes involved in early mammalian development, tissue regeneration and skeletal development; analysis of regulation of Hox homeobox and Sox gene expression and function; inner ear, pancreatic beta-cell, neural crest and heart development.
- Developmental biology of the nervous system; nerve cell membrane functions; role of proteoglycans in neuronal growth and regeneration; hearing and balance defects.
Recombineering - mechanisms and applications:
- Development of technologies for functional genomic studies and gene therapy; understanding of the mechanism underlying single strand oligonucleotide mediated homologous recombination.
- Biochemical studies of male sex gland components, their interaction with sperm, and their effect on embryogenesis.
- Gangliosides as multifunctional biomodulators in signal transduction; structure-function relationship of ganglioside-modulated protein kinases; signaling pathways in cellular stress responses; the metabolism and biological functions of reactive oxygen species.
- Production of therapeutic proteins in transgenic animals; cell and gene-based therapy in animal models.