Transgenic Core Facility - Protocols
In situ hybridisation and immunohistochemistry |Molecular biology | Transgenic production and maintenance | Misc
In situ hybridisation and immunohistochemistry
- Some general tips
- Probe preparation
- Section RNA ISH
- Whole mount RNA ISH
- Wax embedding of embryos and tissue
- Y.ES kit
- Antigen retrieval (Section IHC with notes Donald)
- Immunostaining of frozen sections of mouse embryos
- Section IHC (Woody, Chinese University)
- Whole mount immunostaining (Dave Wilkinson '96)
- Slide preparation
- X-gal antibody
- Taking photos of sections
- Polyclonal type X antibody
- Peter Bruckners new type II ab (not tested)
- Home-made chemiluminescent substrate (ECL)
- Yeast two-hybrid system
- Preparation of nuclear extracts from mouse embryos